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Colon tissues protein samples were prepared with RIPA lysis buffer supplemented with protease inhibitor and phosphatase inhibitor (Beyotime, China) for 30 min on ice (RIPA lysis buffer: protease inhibitor: phosphatase inhibitor = 50:1:1)

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Shabbir S, Khurram E, Moorthi VS, Eissa YTH, Kamal MA, Butler AE