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The Z-track method, displacing the skin laterally by two to three centimetres before insertion and releasing it after withdrawal, seals the needle path and reduces leakage and irritation

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From 10 nM upward it increased force of contraction in a concentration- and time-dependent way, an effect reduced by GLP-1, GIP and glucagon receptor antagonists but not by propranolol, indicating it runs through the peptide's own receptors and the cAMP system rather than beta-adrenoceptors (DOI)