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doi: 10.1067/mpd.2000.102940, PMID: [DOI] [PubMed] [Google Scholar] 40.Thornalley PJ

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The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4°C