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Phenolic compounds profiling by UHPLC-ESI-MS/MS analysis A selection of phenolic compounds were chosen for a thorough quantitative analysis of the extracts using a Sciex 5500 QTrap + mass spectrometer (AB Sciex LLC, Framingham, MA, USA) via Ultra-High-Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry (UHPLC-ESI-MS/MS), equipped with a Turbo V ion-spray source and coupled to an ExionLC AC System, custom-made by Shimadzu (Shimadzu Corporation, Kyoto, Japan) which includes two ExionLC AC pumps, autosampler, controller, degasser and tray

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Morphine induces terminal micro-opioid receptor desensitization by sustained phosphorylation of serine-375