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Representative pictures of untreated, 0–12 h, and 18 h brains support the findings from our absorbance measures (Figure 1b)

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Processing of undifferentiated 3T3-L1 pre-adipocytes (day 0) and differentiated adipocytes (day 10) were optimized for recovery and reproducibility of 1-MNA levels across cultured batches of 3T3-L1 cells (~passages 7–8) and the 1-MNA levels were compared between the pre-adipocytes and adipocytes