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In each well of a poly-L-lysine coated 96-well plate (μclear, GreinerBio, Kremsmünster, Austria), approximately 4,000 cells were seeded in 100 µl Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal calf serum (FCS) and 1% penicillin and streptomycin (P/S)

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It is a synthetic peptide (a long chain of amino acid molecules) and an analog of growth hormone-releasing peptide